杭州浙大迪迅生物基因工程有限公司

　　原標(biāo)題：標(biāo)準(zhǔn)化過(guò)敏原Blo t 12 的免疫學(xué)特征：IgE表位的鑒定
　?、買(mǎi)gE對(duì)標(biāo)準(zhǔn)化過(guò)敏原反應(yīng)的差異可能具有臨床意義;因此，對(duì)其進(jìn)行分析將有助于設(shè)計(jì)出更好的診斷和治療過(guò)敏性呼吸道疾病的方法。已有研究對(duì)螨蟲(chóng)過(guò)敏原中的幾種亞型進(jìn)行了特征描述，但是還沒(méi)有有關(guān)熱帶無(wú)爪螨標(biāo)準(zhǔn)化過(guò)敏原的臨床意義的信息。②評(píng)價(jià)兩種Blo t 12標(biāo)準(zhǔn)化過(guò)敏原的IgE反應(yīng)性的差異。③用ELISA法、皮膚點(diǎn)試驗(yàn)和ELISA交叉抑制法分析了Blo t 12標(biāo)準(zhǔn)化過(guò)敏原的IgE結(jié)合特性。用合成的重疊多肽進(jìn)行表位定位。采用折疊識(shí)別方法對(duì)兩種過(guò)敏原的甲殼素結(jié)合域進(jìn)行建模。④Blo t 12.0101的IgE反應(yīng)頻率和強(qiáng)度大于Blo t 12.0102。在Blo t 12.0101中確定了3個(gè)IgE結(jié)合區(qū);其中一個(gè)包含了Blo t 12.0102中不同的兩個(gè)殘基。這些過(guò)敏原的甲殼素結(jié)合區(qū)模型預(yù)測(cè)，它們具有結(jié)構(gòu)差異，可能影響抗體對(duì)這些表位中的其中一個(gè)的識(shí)別。在Blo t 12生物信息學(xué)的結(jié)構(gòu)分析和免疫學(xué)表征中發(fā)現(xiàn)過(guò)敏原多態(tài)性影響IgE反應(yīng)性。⑤Blo t 12.0101是卡塔赫納地區(qū)最具IgE活性的無(wú)爪螨過(guò)敏原亞型。鑒定出的IgE表位可以經(jīng)過(guò)突變，來(lái)獲得可能用于免疫治療的低過(guò)敏性分子。
　　延伸閱讀
Clin Exp Allergy
Immunological characterization of a Blo t 12 isoallergen: identification of immunoglobulin E epitopes
https://doi.org/10.1111/j.1365-2222.2008.03193.
Abstract:
Background
Differences in the IgE response to isoallergens could have clinical implications; therefore, its analysis will contribute to the design of better strategies for the diagnosis and treatment of allergic respiratory diseases. Several isoforms have been described from mites but there is no information about the clinical impact of Blomia tropicalis isoallergens.
Objective To evaluate the differences in the IgE response against two Blo t 12 isoallergens.
Methods The IgE‐binding properties of Blo t 12 isoallergens were analysed by ELISA, a skin prick test and ELISA cross‐inhibition. Epitope mapping was performed using synthetic overlapping peptides. Fold recognition methods were used to model the chitin‐binding domain of the two isoallergens. Results The frequency and strength of the IgE response were greater for Blo t 12.0101 than for Blo t 12.0102. Three IgE‐binding areas were identified in Blo t 12.0101; one of them included two residues that are different in Blo t 12.0102. Modelling of the chitin‐binding domains of these allergens predicted that they have structural differences that could influence antibody recognition of one of these epitopes. Conclusion In silico structural analysis and immunological characterization of Blo t 12 reveals that allergen polymorphism influences IgE reactivity. Blo t 12.0101 is the most IgE‐reactive isoform in Cartagena. The identified IgE epitopes could be mutated to obtain hypoallergenic molecules of potential use for immunotherapy.
All Authors:
J. Zakzuk S. Jiménez N. Cheong L. Puerta B. W. Lee K. Y. Chua L. Caraballo
　　創(chuàng)建過(guò)敏性疾病的科研、科普知識(shí)交流平臺(tái)，為過(guò)敏患者提供專(zhuān)業(yè)診斷、治療、預(yù)防的共享平臺(tái)。