杭州浙大迪迅生物基因工程有限公司

　　原標題：特應性皮炎患者對各種屋塵螨過敏原中次要組分的敏感性高于呼吸道過敏性疾病患者
　?、偈覂?nèi)塵螨(HDM)可產(chǎn)生多種致敏蛋白。然而，這些過敏原的致敏性和臨床意義尚不清楚。②本研究的目的是確定粉塵螨過敏原，并闡明韓國患有呼吸道(變應性鼻炎和/或哮喘)和特應性皮炎癥狀的患者對這些過敏原的敏感性情況。③對160例HDM過敏患者血清IgE反應性進行單、二維凝膠電泳和免疫印跡分析。IgE‐活性組分采用液相色譜-耦合電噴霧電離-串聯(lián)質(zhì)譜法測定。產(chǎn)生9種重組螨變應原(Der f1、Der f2、Der f10、Der f11、Der f13、Der f14、Der f30、Der f32、Der f Alt a 10)， ELISAs測定血清中IgE對上述組份的反應活性。④ Der f1和Der f2分別被88.1%和78.1%患者的血清IgE識別。呼吸道過敏患者主要對這兩種主要過敏原敏感，特應性皮炎患者對主要和次要過敏原成分(包括Der f11、Der f13、Der f14、Der f32和Der falt a10)表現(xiàn)出多重致敏性。⑤呼吸道過敏性疾病患者對HDM的主要過敏原組份敏感。特應性皮炎患者對HDM中更廣泛的次要過敏原組份(Der f11、Der f13、Der f14、Der f32和Der falt a10)敏感。


延伸閱讀
Clinical & Experimental Allergy：
Sensitization to various minor house dust mite allergens is greater in patients with atopic dermatitis than in those with respiratory allergic disease
https://doi.org/10.1111/cea.13164
Background
Various allergenic proteins are produced by house dust mites (HDM). However, the allergenicity and clinical implications of these allergens are unknown.
Objective
The purpose of this study was to identify allergens in Dermatophagoides farinae and elucidate the sensitization profiles to these in Korean patients suffering from respiratory (allergic rhinitis and/or asthma) and atopic dermatitis symptoms.
Methods
IgE reactivities in sera from 160 HDM allergy patients were analysed by one‐ and two‐dimensional gel electrophoresis and immunoblotting. IgE‐reactive components were identified by liquid chromatography‐coupled electrospray ionization‐tandem mass spectrometry. Nine recombinant mite allergens (Der f 1, Der f 2, Der f 10, Der f 11, Der f 13, Der f 14, Der f 30, Der f 32 and Der f Alt a 10) were produced, and the IgE reactivity in sera to each was determined by ELISAs.
Results
Der f 1 and Der f 2 were recognized by IgE in serum samples from 88.1% and 78.1% of all patients, respectively. Patients with respiratory allergies were mainly sensitized to these major allergens, whereas patients with atopic dermatitis symptoms showed polysensitization to major and minor allergen components (including Der f 11, Der f 13, Der f 14, Der f 32 and Der f Alt a 10).
Conclusions
Patients with respiratory allergic disease sensitize to major allergen components of HDM. Those with atopic dermatitis were sensitized to a broader range of minor allergen components of HDM (Der f 11, Der f 13, Der f 14, Der f 32 and Der f Alt a 10).
All Authors
K. H. Park J. Lee J.‐Y. Lee S. C. LeeD. W. SimJ. U. ShinC. O. ParkJ.‐H. LeeK. H. LeeK. Y. Jeong
　　創(chuàng)建過敏性疾病的科研、科普知識交流平臺，為過敏患者提供專業(yè)診斷、治療、預防的共享平臺。